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Sample GSM1717976 Query DataSets for GSM1717976
Status Public on Jan 15, 2016
Title SKMEL28 control rep4
Sample type RNA
 
Source name cell line
Organism Homo sapiens
Characteristics treatment: control
cell line: SKMEL28
Treatment protocol Cells were treated with a control gapmer or GapmeR3 targeting LINC01212. Alternatively, LINC01212 was overexpressed.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy mini kit (Qiagen)
Label Cy3
Label protocol Total RNA (100 ng) was labeled using the low input Quick Amp WT labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled RNA was hybridized to a Agilent human gene expression microarray according to the manufacturer's instructions.
Scan protocol Arrays were scanned using the Agilent G2565CA Microarray Scanner and Scan Control Software 8.5 according to the manufacturer's instructions.
Data processing Probe intensities were extracted using Feature Extraction software (Agilent).
 
Submission date Jun 23, 2015
Last update date Jan 15, 2016
Contact name Pieter mestdagh
E-mail(s) pieter.mestdagh@ugent.be
Organization name Center for Medical Genetics
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
 
Platform ID GPL20604
Series (2)
GSE70176 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy [1]
GSE70180 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy

Data table header descriptions
ID_REF
VALUE Probe intensities were background corrected and normalized (quantile normalization) using the LIMMA package in R.

Data table
ID_REF VALUE
GE_BrightCorner 16.07261273
DarkCorner 3.746540957
A_33_P3400429 5.887230869
A_33_P3255531 5.012582617
A_24_P940149 7.816342919
A_33_P3306510 10.31526389
CUST_21085_PI427704219 3.981543658
A_33_P3348313 10.45280401
A_33_P3403361 11.29840374
A_33_P3348973 6.918382177
A_23_P11070 3.77756759
A_24_P274615 7.473290049
CUST_1480_PI427704219 4.357772831
CUST_1068_PI427704219 3.683804698
CUST_13414_PI427704219 4.260491034
A_24_P928969 6.891953872
A_23_P100074 9.888383073
A_33_P3227506 4.888631398
CUST_17237_PI427704219 5.914265188
CUST_12234_PI427704219 4.357772831

Total number of rows: 57244

Table truncated, full table size 1616 Kbytes.




Supplementary file Size Download File type/resource
GSM1717976_US45103088_254164810139_S01_GE1_105_Dec08_1_4.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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