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Sample GSM1717974 Query DataSets for GSM1717974
Status Public on Jan 15, 2016
Title SKMEL28 control rep2
Sample type RNA
Source name cell line
Organism Homo sapiens
Characteristics treatment: control
cell line: SKMEL28
Treatment protocol Cells were treated with a control gapmer or GapmeR3 targeting LINC01212. Alternatively, LINC01212 was overexpressed.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy mini kit (Qiagen)
Label Cy3
Label protocol Total RNA (100 ng) was labeled using the low input Quick Amp WT labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
Hybridization protocol Labeled RNA was hybridized to a Agilent human gene expression microarray according to the manufacturer's instructions.
Scan protocol Arrays were scanned using the Agilent G2565CA Microarray Scanner and Scan Control Software 8.5 according to the manufacturer's instructions.
Data processing Probe intensities were extracted using Feature Extraction software (Agilent).
Submission date Jun 23, 2015
Last update date Jan 15, 2016
Contact name Pieter mestdagh
Organization name Center for Medical Genetics
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
Platform ID GPL20604
Series (2)
GSE70176 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy [1]
GSE70180 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy

Data table header descriptions
VALUE Probe intensities were background corrected and normalized (quantile normalization) using the LIMMA package in R.

Data table
GE_BrightCorner 16.36501141
DarkCorner 3.966526628
A_33_P3400429 5.550006833
A_33_P3255531 3.98748766
A_24_P940149 7.946447909
A_33_P3306510 10.3206079
CUST_21085_PI427704219 3.921751646
A_33_P3348313 10.47374753
A_33_P3403361 11.4791338
A_33_P3348973 7.170283094
A_23_P11070 3.600097345
A_24_P274615 6.592178921
CUST_1480_PI427704219 4.047516295
CUST_1068_PI427704219 3.952463938
CUST_13414_PI427704219 4.421361408
A_24_P928969 6.881750884
A_23_P100074 9.789993008
A_33_P3227506 5.195094508
CUST_17237_PI427704219 5.528424791
CUST_12234_PI427704219 3.704401326

Total number of rows: 57244

Table truncated, full table size 1615 Kbytes.

Supplementary file Size Download File type/resource
GSM1717974_US45103088_254164810139_S01_GE1_105_Dec08_1_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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