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Sample GSM1717960 Query DataSets for GSM1717960
Status Public on Jan 15, 2016
Title MM087 control rep2
Sample type RNA
 
Source name short term culture
Organism Homo sapiens
Characteristics treatment: control
cell type: melanoma short term culture
Treatment protocol Cells were treated with a control gapmer or GapmeR3 targeting LINC01212. Alternatively, LINC01212 was overexpressed.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy mini kit (Qiagen)
Label Cy3
Label protocol Total RNA (100 ng) was labeled using the low input Quick Amp WT labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled RNA was hybridized to a Agilent human gene expression microarray according to the manufacturer's instructions.
Scan protocol Arrays were scanned using the Agilent G2565CA Microarray Scanner and Scan Control Software 8.5 according to the manufacturer's instructions.
Data processing Probe intensities were extracted using Feature Extraction software (Agilent).
 
Submission date Jun 23, 2015
Last update date Jan 15, 2016
Contact name Pieter mestdagh
E-mail(s) pieter.mestdagh@ugent.be
Organization name Center for Medical Genetics
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
 
Platform ID GPL20604
Series (2)
GSE70176 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy [1]
GSE70180 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy

Data table header descriptions
ID_REF
VALUE Probe intensities were background corrected and normalized (quantile normalization) using the LIMMA package in R.

Data table
ID_REF VALUE
GE_BrightCorner 16.19445893
DarkCorner 3.984964151
A_33_P3400429 7.65356857
A_33_P3255531 4.526483047
A_24_P940149 5.662951199
A_33_P3306510 11.52126057
CUST_21085_PI427704219 3.688677484
A_33_P3348313 11.1615789
A_33_P3403361 10.9377919
A_33_P3348973 7.195536832
A_23_P11070 3.306829436
A_24_P274615 6.14427889
CUST_1480_PI427704219 4.823111718
CUST_1068_PI427704219 3.422304243
CUST_13414_PI427704219 5.558184
A_24_P928969 7.497616592
A_23_P100074 11.65902002
A_33_P3227506 6.851380881
CUST_17237_PI427704219 6.696784431
CUST_12234_PI427704219 3.808797682

Total number of rows: 57244

Table truncated, full table size 1615 Kbytes.




Supplementary file Size Download File type/resource
GSM1717960_US45103088_254164810138_S01_GE1_105_Dec08_1_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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