|
Status |
Public on Jun 24, 2015 |
Title |
SpeciesMix_FluidigmC1 Cells 80 |
Sample type |
SRA |
|
|
Source name |
HEK and 3T3 mix
|
Organisms |
Homo sapiens; Mus musculus |
Characteristics |
cell line: HEK and 3T3 mix
|
Extracted molecule |
total RNA |
Extraction protocol |
Single cell suspensions were processed through C1 according to manufacturer's instructions Libraries were prepared from C1-mediated whole transcriptome amplifications using Nextera XT (Illumina)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Base calls performed with bcl2fastq2.15 Read 1was aligned with Star v2.4.0 Genome_build: mm10 and hg19 (dual reference) Supplementary_files_format_and_content: bam file
|
|
|
Submission date |
Jun 23, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Evan Macosko |
E-mail(s) |
emacosko@genetics.med.harvard.edu
|
Organization name |
Harvard Medical School
|
Department |
Genetics
|
Lab |
McCarroll Lab
|
Street address |
77 Avenue Louis Pasteur, NRB 260
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL19415 |
Series (2) |
GSE63473 |
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets |
GSE70151 |
C1 analysis using mixtures of human (HEK) and mouse (3T3) cells |
|
Relations |
BioSample |
SAMN03785723 |
SRA |
SRX1069842 |