|Public on Mar 25, 2016
|Jejunum lacking enterocytes
treatment: Bisulfite-converted Padlock
snp c/t-13910: CT
tissue: Jejunum lacking enterocytes
|Human jejunum surgical samples, blood and sperm was examined as well as mouse jejunum
|DNA from tissues were extracted by standard phenol chloroform extraction
Bisulfite padlock probes technique was used to interrogate the lactase gene region and minichromosome maintenance deficient 6 gene of humans and mice
|Illumina HiSeq 2500
|Sample name: H4.4/Ind19
|Analysis of the padlock BS-seq data was completed using a custom pipeline based on the Bismark tool
Reads were first trimmed and residual adapter sequences removed using Trimmomatic-0.32.
Reads that did not align to phiX were aligned to the target reference genome (GRCh37/hg19 or GRCm38/mm10).
Percent modification was estimated as the fraction of spanning reads that retained the reference “C”, and were not converted to “T” from the bisulfite treatment.
Genome_build: hg19 and mm10
Supplementary_files_format_and_content: tab-delimited text files with coverage of modified and unmodified cytosines, with fields: chromosome, position, type, # reads with modified cytosine, # reads with unmodified cytosine
|Jun 18, 2015
|Last update date
|May 15, 2019
|Centre for Addiction and Mental Health
|250 College St Rm606
|Fine-mapping of DNA modifications in the lactase gene region of humans and mice
|Epigenetics of lactose intolerance and lactase persistence