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Status
Public on Feb 21, 2004
Title
Human myometrial cells stably-transfected with the CREM tau2alpha gene
Sample type
RNA
Source name
human myometrium
Organism
Homo sapiens Extracted molecule
total RNA
Description
Human myometrial cells taken from biopsies of pre-menopausal non-cancerous non-pregnant uteri were cultured in complete MEM D-valine medium plus 10% FCS, and stably-transfected with the pcDNA3.1/V5-His TOPO vector (Invitrogen) harbouring the CREM tau2alpha gene. Total RNA was extracted using TRI-reagent (Sigma) and RNeasy columns (Qiagen), and 10 ug used in target preparation for array hybridization.
Submission date
Feb 19, 2004
Last update date
May 28, 2005
Contact name
Jarrod Bailey
E-mail(s)
jarrod.bailey@ncl.ac.uk Phone
(+44) 191 2228748
Fax
(+44) 191 2225066
Organization name
University of Newcastle upon Tyne
Department
School of Surgical and Reproductive Sciences
Street address
Leech Building, Medical School
City
Newcastle upon Tyne
State/province
Tyne and Wear
ZIP/Postal code
NE2 4HH
Country
United Kingdom
Platform ID
GPL96 Series (1)
GSE1059 Myometrial cells expressing CREB, CREM alpha, CREM tau2alpha, ATF2 or the ATF2-small gene
Data table header descriptions ID_REF
VALUE Scaled signal intensity data
ABS_CALL Absolute detection call
Data table ID_REF VALUE ABS_CALL AFFX-BioB-5_at
193.1
P
AFFX-BioB-M_at
299.6
P
AFFX-BioB-3_at
136.5
P
AFFX-BioC-5_at
417.6
P
AFFX-BioC-3_at
321.9
P
AFFX-BioDn-5_at
428.5
P
AFFX-BioDn-3_at
2646.1
P
AFFX-CreX-5_at
4486.1
P
AFFX-CreX-3_at
6779.7
P
AFFX-DapX-5_at
18.7
A
AFFX-DapX-M_at
21.4
A
AFFX-DapX-3_at
1.8
A
AFFX-LysX-5_at
10
A
AFFX-LysX-M_at
27.4
A
AFFX-LysX-3_at
19.1
A
AFFX-PheX-5_at
3.4
A
AFFX-PheX-M_at
1.9
A
AFFX-PheX-3_at
19.5
A
AFFX-ThrX-5_at
4.3
A
AFFX-ThrX-M_at
18.3
A
22283 392 Kbytes .Supplementary data files not provided
