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Sample GSM17040 Query DataSets for GSM17040
Status Public on Feb 21, 2004
Title Control empty-vector stably-transfected human myometrial cells
Sample type RNA
Source name human myometrium
Organism Homo sapiens
Extracted molecule total RNA
Description Human myometrial cells taken from biopsies of pre-menopausal non-cancerous non-pregnant uteri were cultured in complete MEM D-valine medium plus 10% FCS. The cells were stably-transfected with the empty vector pcDNA3.1/V5-His TOPO (Invitrogen) using G418 for selection, as a control against cells stably-transfected with this vector harbouring our genes of interest. Total RNA was extracted using TRI-reagent (Sigma) and RNeasy columns (Qiagen), and 10 ug used in target preparation for array hybridization.
Submission date Feb 18, 2004
Last update date May 28, 2005
Contact name Jarrod Bailey
Phone (+44) 191 2228748
Fax (+44) 191 2225066
Organization name University of Newcastle upon Tyne
Department School of Surgical and Reproductive Sciences
Street address Leech Building, Medical School
City Newcastle upon Tyne
State/province Tyne and Wear
ZIP/Postal code NE2 4HH
Country United Kingdom
Platform ID GPL96
Series (1)
GSE1059 Myometrial cells expressing CREB, CREM alpha, CREM tau2alpha, ATF2 or the ATF2-small gene

Data table header descriptions
VALUE Scaled signal intensity data
ABS_CALL Absolute detection call

Data table
AFFX-BioB-5_at 157.8 P
AFFX-BioB-M_at 208.2 P
AFFX-BioB-3_at 108.8 P
AFFX-BioC-5_at 359.7 P
AFFX-BioC-3_at 246.1 P
AFFX-BioDn-5_at 323.1 P
AFFX-BioDn-3_at 2504.3 P
AFFX-CreX-5_at 3634.9 P
AFFX-CreX-3_at 5616.1 P
AFFX-DapX-5_at 17.7 A
AFFX-DapX-M_at 7.9 A
AFFX-DapX-3_at 3.7 A
AFFX-LysX-5_at 1.7 A
AFFX-LysX-M_at 3.8 A
AFFX-LysX-3_at 5.4 A
AFFX-PheX-5_at 1.7 A
AFFX-PheX-M_at 7.3 A
AFFX-PheX-3_at 23.5 A
AFFX-ThrX-5_at 1.6 A
AFFX-ThrX-M_at 3.4 A

Total number of rows: 22283

Table truncated, full table size 392 Kbytes.

Supplementary data files not provided

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