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Sample GSM1700757 Query DataSets for GSM1700757
Status Public on May 27, 2021
Title JCYL004D
Sample type SRA
 
Source name Aerobically xylosextrins-grown cells
Organism Saccharomyces cerevisiae
Characteristics strain: Engineered SR8U containing cellobiose-utilizing, xylose-utilizing and xylodextrin-degrading pathways
growth condition: Aerobically
carbon source: Xylodextrins
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RiboPure Yeast kit (Ambion, Austin, TX, USA), according to manufacturer's instructions, except cells were disrupted by bead-beating 3 times for 30 sec, with a 30 sec pause between runs.
4 µg of total RNA were used to prepare the multiplexing libraries with barcodes following the standard instructions of the Illumina TruSeq RNA Sample Prep Kit.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer II
 
Data processing Illumina Casava_v1.8.0 software used for basecalling. Sequenced reads were trimmed for adaptor sequence.
Sequence reads were assembled and analyzed in CLC Genomics Workbench 7.0 (CLC Bio, Aarhus, Denmark). The Saccharomyces cerevisiae S288C genome was downloaded from RefSeq at the NCBI (sequence assembly version R64-1-1, http://www.ncbi.nlm.nih.gov/assembly/GCF_000146045.2/) including 16 chromosomes and the mitochondrial genome. The genes for a cellodextrin transporter gene (cdt-1) and an intracellular β-glucosidase gene (codon-optimized gh1-1) from Neurospora crassa; XYL1 (xylose reductase gene), XYL2 (xylitol dehydrogenase gene), and XKS1 (xylulose kinase gene) from Scheffersomyces stipitis, as well as cdt-2 (coding for cellodextrin transporter 2), gh43-2 (coding for β-xylosidase) and gh43-7 (coding for a xylosyl-xylitol-specific β-xylosidase) from N. crassa were manually annotated and combined with the S. cerevisiae S288C genome as the reference. Expression values were normalized by calculating the reads per kilobase of mRNA exon per million mapped reads (RPKM), and further normalized using the option of “By totals”.
Genome_build: GCF_000146045.2
Supplementary_files_format_and_content: Excel file includes RPKM values for all the samples.
 
Submission date May 31, 2015
Last update date May 27, 2021
Contact name Yuping Lin
E-mail(s) lin_yp@tib.cas.cn
Organization name Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
Street address 32 West 7th Avenue, Tianjin Airport Economic Area
City Tianjin
State/province Tianjin
ZIP/Postal code 300308
Country China
 
Platform ID GPL9377
Series (1)
GSE69404 Impact of plant cell wall derived sugars on transcriptional and post-transcriptional control in Saccharomyces cerevisiae
Relations
BioSample SAMN03753672
SRA SRX1044384

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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