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Sample GSM1697203 Query DataSets for GSM1697203
Status Public on Jul 16, 2015
Title q27_0
Sample type SRA
 
Source name MEF-depleted hiF-T cells at day 10 of reprogramming
Organism Homo sapiens
Characteristics well number: 84
reprogramming time point (day): 10
Growth protocol Refer to the section Cell Culture and Reprogramming in the Supplemental Experimental Procedures of the related manuscript.
Extracted molecule total RNA
Extraction protocol The single-cell RNA-seq libraries were prepared from MEF-depleted hiF-T cells at day 10 of reprogramming directly sorted in lysis buffer at single cell using an ARIA facs sorter (BD).
Library preparation was performed using SMART-seq2 protocol with minor modifications (Trombetta et al., 2014) on 96 cells
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description 10days84
Data processing Data processing was performed using the Tuxedo RNAseq suite (Trapnell et al., 2012). 52 single cells displayed more than 50% alignment rate and 100,000 reads per sample and were therefore considered for further analysis.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample
 
Submission date May 27, 2015
Last update date May 15, 2019
Contact name Shannan J Ho Sui
E-mail(s) shosui@hsph.harvard.edu
Organization name Harvard T.H. han Scholol of Public Health
Department Biostatistics
Street address 655 Huntington Ave, Building 2, Rm 437A
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
 
Platform ID GPL16791
Series (2)
GSE62777 Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency
GSE69292 Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency [smartseq2]
Relations
BioSample SAMN03737856
SRA SRX1040910

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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