|
Status |
Public on Jul 16, 2015 |
Title |
q10_0 |
Sample type |
SRA |
|
|
Source name |
MEF-depleted hiF-T cells at day 10 of reprogramming
|
Organism |
Homo sapiens |
Characteristics |
well number: 61 reprogramming time point (day): 10
|
Growth protocol |
Refer to the section Cell Culture and Reprogramming in the Supplemental Experimental Procedures of the related manuscript.
|
Extracted molecule |
total RNA |
Extraction protocol |
The single-cell RNA-seq libraries were prepared from MEF-depleted hiF-T cells at day 10 of reprogramming directly sorted in lysis buffer at single cell using an ARIA facs sorter (BD). Library preparation was performed using SMART-seq2 protocol with minor modifications (Trombetta et al., 2014) on 96 cells
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
10days61
|
Data processing |
Data processing was performed using the Tuxedo RNAseq suite (Trapnell et al., 2012). 52 single cells displayed more than 50% alignment rate and 100,000 reads per sample and were therefore considered for further analysis. Genome_build: hg19 Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample
|
|
|
Submission date |
May 27, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Shannan Ho Sui |
E-mail(s) |
shosui@hsph.harvard.edu
|
Organization name |
Harvard T.H. Chan School of Public Health
|
Department |
Biostatistics
|
Street address |
401 Park Drive, Level 3 East
|
City |
Boston |
State/province |
Massachusetts |
ZIP/Postal code |
02215 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (2) |
GSE62777 |
Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency |
GSE69292 |
Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency [smartseq2] |
|
Relations |
BioSample |
SAMN03737868 |
SRA |
SRX1040893 |