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Sample GSM1694975 Query DataSets for GSM1694975
Status Public on Jul 16, 2015
Title hIPSC17a hIPSC
Sample type SRA
Source name reference hIPSC line
Organism Homo sapiens
Characteristics cell line: hIPSC17a
Growth protocol Refer to the section Cell Culture and Reprogramming in the Supplemental Experimental Procedures of the related manuscript.
Extracted molecule total RNA
Extraction protocol Total RNA, including the small RNA fraction, was obtained by organic extraction followed by miRNeasy purification (Qiagen).
RNA-Seq Illumina libraries for mRNA profiling were prepared from 100 ng total RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina).
TruSeq RNA Sample Prep Kit v2 (Illumina)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing Reads were mapped with TopHat version 2.0.7 and Bowtie version 2.1.0 with default parameters against build hg19 of the human genome, and build 13 of the GENCODE human genome annotation. Samples were quantified with the Cufflinks package version 2.2.0. Differential expression was performed using Cuffdiff 2.2.0 with default parameters.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
Submission date May 22, 2015
Last update date May 15, 2019
Contact name Shannan J Ho Sui
Organization name Harvard T.H. han Scholol of Public Health
Department Biostatistics
Street address 655 Huntington Ave, Building 2, Rm 437A
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
Platform ID GPL11154
Series (2)
GSE62772 Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency [mRNA-Seq]
GSE62777 Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency
BioSample SAMN03732538
SRA SRX1037215

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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