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Sample GSM1693951 Query DataSets for GSM1693951
Status Public on Sep 01, 2016
Title 40_U2OS_3d_HINFP_2
Sample type RNA
 
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: HINFP
day after sirna treatment: Day 3
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after HINFP siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 9.234434148
ENSG00000000005_at 3.270815641
ENSG00000000419_at 10.68478034
ENSG00000000457_at 6.558254835
ENSG00000000460_at 7.684409793
ENSG00000000938_at 4.694816176
ENSG00000000971_at 3.355517544
ENSG00000001036_at 9.297703927
ENSG00000001084_at 8.716464154
ENSG00000001167_at 9.851238603
ENSG00000001460_at 7.644941731
ENSG00000001461_at 7.713276464
ENSG00000001497_at 9.497791574
ENSG00000001561_at 6.222302636
ENSG00000001617_at 6.790174983
ENSG00000001626_at 3.149652576
ENSG00000001629_at 10.60686529
ENSG00000001631_at 8.252637369
ENSG00000002016_at 6.879007312
ENSG00000002079_at 7.143106737

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693951_40_U2OS_HINFP_2_F05.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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