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Sample GSM1693950 Query DataSets for GSM1693950
Status Public on Sep 01, 2016
Title 39_U2OS_3d_HINFP_1
Sample type RNA
 
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: HINFP
day after sirna treatment: Day 3
replica: biological rep 1
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after HINFP siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 9.236782534
ENSG00000000005_at 3.335838268
ENSG00000000419_at 10.7423637
ENSG00000000457_at 6.769409634
ENSG00000000460_at 7.955618553
ENSG00000000938_at 4.833126204
ENSG00000000971_at 3.4319776
ENSG00000001036_at 9.218342985
ENSG00000001084_at 8.701959287
ENSG00000001167_at 9.690702338
ENSG00000001460_at 7.350639226
ENSG00000001461_at 7.507896933
ENSG00000001497_at 9.501995554
ENSG00000001561_at 5.867124005
ENSG00000001617_at 6.670829283
ENSG00000001626_at 3.263607105
ENSG00000001629_at 10.49275489
ENSG00000001631_at 8.496779218
ENSG00000002016_at 7.047151034
ENSG00000002079_at 6.832160217

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693950_39_U2OS_HINFP_1_G09.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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