GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1693947 Query DataSets for GSM1693947
Status Public on Sep 01, 2016
Title 36_U2OS_3d_CASP8AP2_2
Sample type RNA
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: CASP8AP2
day after sirna treatment: Day 3
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 9.003363669
ENSG00000000005_at 3.314177976
ENSG00000000419_at 10.58832225
ENSG00000000457_at 6.490715451
ENSG00000000460_at 7.500826433
ENSG00000000938_at 4.776138635
ENSG00000000971_at 3.546650523
ENSG00000001036_at 9.377378183
ENSG00000001084_at 8.623690427
ENSG00000001167_at 9.912300032
ENSG00000001460_at 7.656640317
ENSG00000001461_at 7.500158689
ENSG00000001497_at 9.596717378
ENSG00000001561_at 5.264908614
ENSG00000001617_at 6.481156147
ENSG00000001626_at 3.166386663
ENSG00000001629_at 10.2698687
ENSG00000001631_at 8.53175794
ENSG00000002016_at 6.945522826
ENSG00000002079_at 7.003868179

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693947_36_U2OS_FLASH_2_B05.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap