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Sample GSM1693946 Query DataSets for GSM1693946
Status Public on Sep 01, 2016
Title 35_U2OS_3d_CASP8AP2_1
Sample type RNA
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: CASP8AP2
day after sirna treatment: Day 3
replica: biological rep 1
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 8.835310379
ENSG00000000005_at 3.571039938
ENSG00000000419_at 10.41817228
ENSG00000000457_at 6.308083964
ENSG00000000460_at 7.525014141
ENSG00000000938_at 4.885543589
ENSG00000000971_at 3.625593999
ENSG00000001036_at 9.234269863
ENSG00000001084_at 8.581562966
ENSG00000001167_at 9.750961247
ENSG00000001460_at 7.579316638
ENSG00000001461_at 7.169525141
ENSG00000001497_at 9.692446587
ENSG00000001561_at 5.334924755
ENSG00000001617_at 6.139101646
ENSG00000001626_at 3.253425914
ENSG00000001629_at 10.20834516
ENSG00000001631_at 8.42811303
ENSG00000002016_at 6.818221823
ENSG00000002079_at 7.050189415

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693946_35_U2OS_FLASH_1_D07.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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