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Sample GSM1693945 Query DataSets for GSM1693945
Status Public on Sep 01, 2016
Title 34_U2OS_3d_CONT_2
Sample type RNA
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: control
day after sirna treatment: Day 3
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after control siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 9.335903239
ENSG00000000005_at 3.461699955
ENSG00000000419_at 10.87887567
ENSG00000000457_at 6.825826031
ENSG00000000460_at 7.987133039
ENSG00000000938_at 4.456841025
ENSG00000000971_at 3.881756584
ENSG00000001036_at 9.676249687
ENSG00000001084_at 8.750340227
ENSG00000001167_at 10.03901121
ENSG00000001460_at 8.050467121
ENSG00000001461_at 7.452978169
ENSG00000001497_at 9.684284524
ENSG00000001561_at 5.471717316
ENSG00000001617_at 6.385384917
ENSG00000001626_at 3.16361214
ENSG00000001629_at 10.38633779
ENSG00000001631_at 8.436505052
ENSG00000002016_at 7.42177658
ENSG00000002079_at 7.185883956

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693945_34_U2OS_CONT_2_F09.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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