GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1693938 Query DataSets for GSM1693938
Status Public on Sep 01, 2016
Title 27_RPE_3d_CASP8AP2_1
Sample type RNA
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: CASP8AP2
day after sirna treatment: Day 3
replica: biological rep 1
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 8.473062888
ENSG00000000005_at 3.477061792
ENSG00000000419_at 10.15436798
ENSG00000000457_at 6.743331794
ENSG00000000460_at 7.458876291
ENSG00000000938_at 4.65751951
ENSG00000000971_at 8.137036137
ENSG00000001036_at 9.927192853
ENSG00000001084_at 7.554863449
ENSG00000001167_at 8.537833828
ENSG00000001460_at 8.312296477
ENSG00000001461_at 8.610239135
ENSG00000001497_at 8.266579618
ENSG00000001561_at 4.201149187
ENSG00000001617_at 8.142893128
ENSG00000001626_at 3.149950566
ENSG00000001629_at 10.20423001
ENSG00000001631_at 7.867456226
ENSG00000002016_at 6.472579554
ENSG00000002079_at 6.047875474

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693938_27_RPE_FLASH_1_A09.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap