NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1693937 Query DataSets for GSM1693937
Status Public on Sep 01, 2016
Title 26_RPE_3d_CONT_2
Sample type RNA
 
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: control
day after sirna treatment: Day 3
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after control siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 8.35046068
ENSG00000000005_at 3.553246035
ENSG00000000419_at 9.838573679
ENSG00000000457_at 6.938045212
ENSG00000000460_at 7.347336965
ENSG00000000938_at 4.81524197
ENSG00000000971_at 8.378730583
ENSG00000001036_at 10.04337989
ENSG00000001084_at 7.37276652
ENSG00000001167_at 8.866822371
ENSG00000001460_at 7.926368837
ENSG00000001461_at 8.36582625
ENSG00000001497_at 8.494436131
ENSG00000001561_at 4.847786384
ENSG00000001617_at 7.907831331
ENSG00000001626_at 3.299293776
ENSG00000001629_at 9.941913313
ENSG00000001631_at 7.900330757
ENSG00000002016_at 6.675571365
ENSG00000002079_at 5.047200179

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693937_26_RPE_CONT_2_A05.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap