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Sample GSM1693936 Query DataSets for GSM1693936
Status Public on Sep 01, 2016
Title 25_RPE_3d_CONT_1
Sample type RNA
 
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: control
day after sirna treatment: Day 3
replica: biological rep 1
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after control siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 8.545198709
ENSG00000000005_at 3.634739366
ENSG00000000419_at 9.97110051
ENSG00000000457_at 6.808159404
ENSG00000000460_at 7.442030165
ENSG00000000938_at 4.794921534
ENSG00000000971_at 8.040980099
ENSG00000001036_at 9.709921832
ENSG00000001084_at 7.200327654
ENSG00000001167_at 8.377975282
ENSG00000001460_at 8.312640976
ENSG00000001461_at 8.262880854
ENSG00000001497_at 8.193335235
ENSG00000001561_at 4.495985934
ENSG00000001617_at 8.146784026
ENSG00000001626_at 3.217037849
ENSG00000001629_at 9.962468317
ENSG00000001631_at 7.810031036
ENSG00000002016_at 6.375206845
ENSG00000002079_at 5.391854216

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693936_25_RPE_CONT_1_B09.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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