GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1693935 Query DataSets for GSM1693935
Status Public on Sep 01, 2016
Title 24_U2OS_2d_CASP8AP2_3
Sample type RNA
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: CASP8AP2
day after sirna treatment: Day 2
replica: biological rep 3
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 2nd day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 9.405238595
ENSG00000000005_at 3.302361343
ENSG00000000419_at 11.66310197
ENSG00000000457_at 6.877823488
ENSG00000000460_at 7.672720734
ENSG00000000938_at 4.843089547
ENSG00000000971_at 3.378276388
ENSG00000001036_at 10.11626168
ENSG00000001084_at 8.679719892
ENSG00000001167_at 9.989190185
ENSG00000001460_at 8.21615605
ENSG00000001461_at 8.286097984
ENSG00000001497_at 9.796658157
ENSG00000001561_at 6.213683161
ENSG00000001617_at 6.779464647
ENSG00000001626_at 3.132181777
ENSG00000001629_at 10.64175689
ENSG00000001631_at 8.808988184
ENSG00000002016_at 7.494533583
ENSG00000002079_at 7.489887589

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693935_24_U2OS_2d_FLASH_3.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap