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Sample GSM1693931 Query DataSets for GSM1693931
Status Public on Sep 01, 2016
Title 20_U2OS_2d_control_2
Sample type RNA
 
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: control
day after sirna treatment: Day 2
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 2nd day after control siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 9.491794908
ENSG00000000005_at 3.130178162
ENSG00000000419_at 11.76888608
ENSG00000000457_at 6.855992503
ENSG00000000460_at 7.935219584
ENSG00000000938_at 4.757674584
ENSG00000000971_at 3.574720132
ENSG00000001036_at 10.06666286
ENSG00000001084_at 8.745090283
ENSG00000001167_at 10.19407323
ENSG00000001460_at 8.161301307
ENSG00000001461_at 7.73488315
ENSG00000001497_at 10.22952082
ENSG00000001561_at 6.371352245
ENSG00000001617_at 6.462754549
ENSG00000001626_at 3.008248977
ENSG00000001629_at 10.63414053
ENSG00000001631_at 8.838356283
ENSG00000002016_at 7.425484283
ENSG00000002079_at 7.410949866

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693931_20_U2OS_2d_control_2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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