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Sample GSM1693928 Query DataSets for GSM1693928
Status Public on Sep 01, 2016
Title 17_U2OS_1d_CASP8AP2_2
Sample type RNA
 
Source name U2OS
Organism Homo sapiens
Characteristics cell line: U2OS
sirna: CASP8AP2
day after sirna treatment: Day 1
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 1st day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 9.229729007
ENSG00000000005_at 3.213675943
ENSG00000000419_at 11.67756158
ENSG00000000457_at 6.861539046
ENSG00000000460_at 7.675737881
ENSG00000000938_at 4.541451654
ENSG00000000971_at 3.567202332
ENSG00000001036_at 10.06360123
ENSG00000001084_at 8.717986816
ENSG00000001167_at 10.27904678
ENSG00000001460_at 8.273001651
ENSG00000001461_at 7.284195592
ENSG00000001497_at 10.04751576
ENSG00000001561_at 6.143463714
ENSG00000001617_at 6.708907472
ENSG00000001626_at 2.979408963
ENSG00000001629_at 10.67203229
ENSG00000001631_at 8.872491557
ENSG00000002016_at 7.512966185
ENSG00000002079_at 7.139682734

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693928_17_U2OS_1d_FLASH_2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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