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Sample GSM1693921 Query DataSets for GSM1693921
Status Public on Sep 01, 2016
Title 10_RPE1_2d_CASP8AP2_1
Sample type RNA
 
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: CASP8AP2
day after sirna treatment: Day 2
replica: biological rep 1
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 2nd day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 8.686141817
ENSG00000000005_at 3.241909798
ENSG00000000419_at 11.17587311
ENSG00000000457_at 7.128950216
ENSG00000000460_at 7.521107791
ENSG00000000938_at 4.97546449
ENSG00000000971_at 8.295353544
ENSG00000001036_at 10.69352308
ENSG00000001084_at 7.465975092
ENSG00000001167_at 9.277613812
ENSG00000001460_at 8.657691644
ENSG00000001461_at 8.9660115
ENSG00000001497_at 8.935255121
ENSG00000001561_at 5.357809016
ENSG00000001617_at 8.466862375
ENSG00000001626_at 3.048770114
ENSG00000001629_at 10.38908058
ENSG00000001631_at 8.231619912
ENSG00000002016_at 6.852230853
ENSG00000002079_at 5.627685532

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693921_10_RPE1_2d_FLASH_1.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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