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Sample GSM1693920 Query DataSets for GSM1693920
Status Public on Sep 01, 2016
Title 09_RPE1_2d_control_3
Sample type RNA
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: control
day after sirna treatment: Day 2
replica: biological rep 3
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 2nd day after control siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 8.595977547
ENSG00000000005_at 3.698752765
ENSG00000000419_at 11.24182957
ENSG00000000457_at 7.394726772
ENSG00000000460_at 7.836241459
ENSG00000000938_at 5.065864992
ENSG00000000971_at 8.519515525
ENSG00000001036_at 10.62106016
ENSG00000001084_at 7.35912683
ENSG00000001167_at 9.514159018
ENSG00000001460_at 8.461583582
ENSG00000001461_at 9.057570118
ENSG00000001497_at 8.872442128
ENSG00000001561_at 5.456176395
ENSG00000001617_at 8.143578149
ENSG00000001626_at 3.024037846
ENSG00000001629_at 10.38484283
ENSG00000001631_at 8.427431171
ENSG00000002016_at 7.293854298
ENSG00000002079_at 5.012204852

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693920_09_RPE1_2d_control_3.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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