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Sample GSM1693917 Query DataSets for GSM1693917
Status Public on Sep 01, 2016
Title 06_RPE1_1d_CASP8AP2_3
Sample type RNA
 
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: CASP8AP2
day after sirna treatment: Day 1
replica: biological rep 3
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 1st day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
 
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
E-mail(s) maria.fulop@helsinki.fi
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
ENSG00000000003_at 8.509005544
ENSG00000000005_at 3.343426023
ENSG00000000419_at 11.26685061
ENSG00000000457_at 7.184206207
ENSG00000000460_at 7.933776867
ENSG00000000938_at 4.900496124
ENSG00000000971_at 8.188450782
ENSG00000001036_at 10.61588613
ENSG00000001084_at 7.509345627
ENSG00000001167_at 9.40057791
ENSG00000001460_at 8.685125727
ENSG00000001461_at 9.013904389
ENSG00000001497_at 8.996858317
ENSG00000001561_at 4.868314907
ENSG00000001617_at 8.004616224
ENSG00000001626_at 3.036840959
ENSG00000001629_at 10.33746905
ENSG00000001631_at 8.455393369
ENSG00000002016_at 6.925920341
ENSG00000002079_at 5.870783289

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1693917_06_RPE1_1d_FLASH_3.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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