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Sample GSM1693916 Query DataSets for GSM1693916
Status Public on Sep 01, 2016
Title 05_RPE1_1d_CASP8AP2_2
Sample type RNA
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: CASP8AP2
day after sirna treatment: Day 1
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 1st day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 8.687351929
ENSG00000000005_at 3.524027393
ENSG00000000419_at 11.19713743
ENSG00000000457_at 7.139877586
ENSG00000000460_at 7.956356714
ENSG00000000938_at 5.157990976
ENSG00000000971_at 7.938498336
ENSG00000001036_at 10.61303606
ENSG00000001084_at 7.612150523
ENSG00000001167_at 9.189528704
ENSG00000001460_at 8.854554968
ENSG00000001461_at 8.926470057
ENSG00000001497_at 8.78159545
ENSG00000001561_at 5.106175809
ENSG00000001617_at 7.803893102
ENSG00000001626_at 3.128780799
ENSG00000001629_at 10.29551984
ENSG00000001631_at 8.440579926
ENSG00000002016_at 6.722527933
ENSG00000002079_at 5.879719211

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693916_05_RPE1_1d_FLASH_2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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