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Sample GSM1693915 Query DataSets for GSM1693915
Status Public on Sep 01, 2016
Title 04_RPE1_1d_CASP8AP2_1
Sample type RNA
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: CASP8AP2
day after sirna treatment: Day 1
replica: biological rep 1
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 1st day after CASP8AP2 siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 8.484131364
ENSG00000000005_at 3.369872617
ENSG00000000419_at 11.32184702
ENSG00000000457_at 7.297079135
ENSG00000000460_at 7.96280728
ENSG00000000938_at 4.819354033
ENSG00000000971_at 8.060506828
ENSG00000001036_at 10.54537931
ENSG00000001084_at 7.591992947
ENSG00000001167_at 9.295828724
ENSG00000001460_at 8.794749518
ENSG00000001461_at 9.027410174
ENSG00000001497_at 9.079591946
ENSG00000001561_at 5.039807481
ENSG00000001617_at 7.961579594
ENSG00000001626_at 3.071494584
ENSG00000001629_at 10.25632352
ENSG00000001631_at 8.37056279
ENSG00000002016_at 6.950002821
ENSG00000002079_at 5.890129565

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693915_04_RPE1_1d_FLASH_1.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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