|
| Status |
Public on May 01, 2018 |
| Title |
Lesional dermis from psoriatic skin, sample 10, set 2 |
| Sample type |
RNA |
| |
|
| Source name |
Human lesional psoriatic dermis (laser capture microdissection), sample 10
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: psoriasis
tissue: dermis
gender: male
age: 51y
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Arcturus Picopure RNA isolation Kit according to manufacturer's protocol
|
| Label |
Cy3
|
| Label protocol |
10 ng RNA were amplified with ExpressArt Kit and labeled (Cy3) with One-color microarray-based Gene Expression Analysis Low Input Quick Amp WT Labeling (Agilent Technologies) according to manufacturer's protocol
|
| |
|
| Hybridization protocol |
1.5 ug of Cy3-labelled cRNA was hybridized with Human Gene Expression Microarray 8x60K chip (Agilent Technologies) according to manufacturer's protocol
|
| Scan protocol |
Slides were scanned with the Agilent G2505B scanner.
|
| Description |
Gene expression in human lesional psoriatic dermis
PN10DL
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version V.10.7.3.1). Data were background-corrected with 'normexp' option and quantile normalized using limma R package.
|
| |
|
| Submission date |
May 15, 2015 |
| Last update date |
May 01, 2018 |
| Contact name |
Denis A Mogilenko |
| Organization name |
INSERM
|
| Department |
U1011
|
| Street address |
1 Rue du Professeur Calmette, BP245
|
| City |
Lille |
| ZIP/Postal code |
59019 |
| Country |
France |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE68939 |
Gene epression in healthy and lesional psoriatic dermis from human skin biopsies (laser capture microdissection): set 2 |
|
