|
| Status |
Public on Jul 31, 2015 |
| Title |
Epidermis, separated from VDR knockout (KO) skin |
| Sample type |
RNA |
| |
|
| Source name |
Skin, epidermis separated from dermis by diapase treatment of the back skin
|
| Organism |
Mus musculus |
| Characteristics |
age: P5
genotype: VDR fl/fl Cre K14 driven
strain: C57BL6
tissue: skin
|
| Treatment protocol |
K14CreVDR KO mice and their littermate control mice at P5 were sacrified. The genotypes were determined by extacting tail DNA by using PCR. The back skin was excised from mice and treated with dispase. The epidermis was separated from dermis. RNA was extracted from epidermis of each mice. The gene expression for specific genes were evaluated by reverse transcription and QPCR to confirm that the variation of gene expression is small within the group. The RNA was combined from 3 mice for CON and VDR KO group.
|
| Growth protocol |
Vitamin D receptor (VDR) is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Stat60 reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
combined triplicates of VDR KO epidermis
|
| Data processing |
data were normalized by using quantile method in Genome studio V2011.1
|
| |
|
| Submission date |
May 11, 2015 |
| Last update date |
Jul 31, 2015 |
| Contact name |
Yuko Oda |
| E-mail(s) |
yuko.oda@ucsf.edu
|
| Phone |
4152692370
|
| Organization name |
VA Medical Center UCSF
|
| Department |
Endocrine Research
|
| Street address |
111N-MB 360 1700 Owens St, Suite 360
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE68723 |
Synergism of calcium sensing receptor and vitamin D receptor mediated signaling in epidermal differentiation [VDR KO] |
| GSE68726 |
Synergism of calcium sensing receptor and vitamin D receptor mediated signaling in epidermal differentiation |
|
