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Sample GSM1664552 Query DataSets for GSM1664552
Status Public on Jun 02, 2015
Title Liver_ko_3
Sample type RNA
 
Channel 1
Source name Liver_ko
Organism Mus musculus
Characteristics genotype: GCGR ko/ko
Sex: Female
tissue: liver
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse livers using Qiagen RNAeasy Lipid Tissue Kit with in-column DNase I treatment.
Label Cy5
Label protocol Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal mouse reference.
 
Channel 2
Source name Universal mouse reference (UMR) from Stratagene
Organism Mus musculus
Characteristics sample type: pooled mouse reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse livers using Qiagen RNAeasy Lipid Tissue Kit with in-column DNase I treatment.
Label Cy3
Label protocol Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal mouse reference.
 
 
Hybridization protocol Agilent In Situ Hybridization Kit Plus was used where Cy-dye labeled control and test samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, the arrays were washed twice, dried and then scanned.
Scan protocol Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software version 10.7.
Data processing Agilent Feature Extraction Software version 10.7 was used for background subtraction and LOWESS normalization.
 
Submission date Apr 21, 2015
Last update date Jun 02, 2015
Contact name Matthew Brauer
E-mail(s) brauer.matthew@gene.com
Organization name Genentech, Inc.
Department Bioinformatics
Street address 1 DNA Way
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
 
Platform ID GPL7202
Series (2)
GSE68143 Glucagon receptor signaling in GCGR KO mice
GSE68145 Glucagon Couples Hepatic Amino Acid Catabolism to mTOR-dependent Regulation of α-cell Mass

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
A_52_P616356 -0.024022459
A_52_P580582 -0.301650088
A_52_P403405 -0.017835001
A_52_P819156 0.11575738
A_51_P331831 -1.372586758
A_51_P430630 -0.346057643
A_52_P502357 0.00351526
A_52_P299964 -0.101211727
A_51_P356389 -0.094674618
A_52_P684402 -0.403634499
A_51_P414208 0.176672552
A_51_P280918 -0.263233466
A_52_P613688 -1.212838145
A_52_P258194 -0.927012656
A_52_P229271 -0.207865698
A_52_P214630 -0.624640585
A_52_P579519 0.481118692
A_52_P979997 -0.050215835
A_52_P453864 -0.126092371
A_52_P655842 0.032449529

Total number of rows: 41174

Table truncated, full table size 1018 Kbytes.




Supplementary file Size Download File type/resource
GSM1664552_Agilent_251486833867_S01_GE2_107_Sep09_1_1.txt.gz 14.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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