NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1660664 Query DataSets for GSM1660664
Status Public on Sep 30, 2015
Title Dox(-) - siAgo2
Sample type RNA
 
Source name HeLa-shDicer cells treated with siAgo2 and without Dox
Organism Homo sapiens
Characteristics cell line: HeLa cells inducibly expressing shDicer under control of Doxycycline (HeLa-shDicer cells)
agent: no Dox
genotype/variation: siAgo2
Treatment protocol The cells were transfected with siControl or siAgp2. After 48 h incubation, total RNA was extracted using Isogen (Nippongen).
Growth protocol Hela-shDicer cells, which inducibly express shDicer by Dox treatment, were cultured in Dox-containing medium.
Extracted molecule total RNA
Extraction protocol Isogen extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated samples were prepared using FlashTag Biotin HSR RNA labeling Kit for Affymetrix GeneChip miRNA Arrays according the the user manual.
 
Hybridization protocol The samples were hybridized for 18 hr on GeneChip miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Data processing The normalization of RMA was performed using Affymetrix Expression Console Software.
 
Submission date Apr 17, 2015
Last update date Sep 30, 2015
Contact name Mitsuhiro Machitani
E-mail(s) mmachita@ncc.go.jp
Phone 081-3-3547-5201
Organization name National Cancer Center Research Institute
Lab Division of Cancer Stem Cell
Street address 5-1-1 Tsukiji
City Chuo-ku
State/province Tokyo
ZIP/Postal code 104-0045
Country Japan
 
Platform ID GPL19117
Series (1)
GSE67992 Expression data from Dicer and Ago2-knockdown HeLa cells

Data table header descriptions
ID_REF
VALUE RMA normalized intensity

Data table
ID_REF VALUE
MIMAT0000001_st 6526.671
MIMAT0015091_st 1.260421
MIMAT0000002_st 1.432799
MIMAT0015092_st 3.076328
MIMAT0020301_st 1.625271
MIMAT0000003_st 1.661248
MIMAT0020302_st 2.21127
MIMAT0000004_st 1.315857
MIMAT0000005_st 1.772959
MIMAT0015093_st 2.637125
MIMAT0020303_st 1.106815
MIMAT0000006_st 1.735767
MIMAT0020304_st 1.315857
MIMAT0000007_st 1.598051
MIMAT0015094_st 1.370033
MIMAT0000008_st 1.315857
MIMAT0020305_st 1.729645
MIMAT0000009_st 2.21127
MIMAT0020306_st 1.370033
MIMAT0000010_st 1.322595

Total number of rows: 36249

Table truncated, full table size 874 Kbytes.




Supplementary file Size Download File type/resource
GSM1660664_2_miRNA-4_0_.CEL.gz 723.1 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap