NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1659025 Query DataSets for GSM1659025
Status Public on Aug 05, 2015
Title Progenitors from patient with predisposition JAK2V617F and TET2 mutation
Sample type genomic
 
Channel 1
Source name Blood
Organism Homo sapiens
Characteristics gender: F
pathology: Essential thrombocythemia
cell status: Primary cells (CD34+)
biosource: hematopoieitc progenitors amplified 7 days with a cocktail of cytokines
Growth protocol IPSC (passage between P15 and P33) were cultured on MEF layers in the presence of FGF2
Extracted molecule genomic DNA
Extraction protocol DNA extraction was performed using the DNEasy Mini Kit (Qiagen). DNA was purified using the ChargeSwitch Forensic DNA Purification Kit (Invitrogen, Carlsberg, CA). Concentration and purity of DNA was determined using a Qubit dsDNA BR kit (Life Technologies).
Label Cy5
Label protocol DNA was digested with AluI and RsaI restriction enzymes. Sample DNA was labeled with Cy5-dUTP, whereas sex-matched normal reference DNA from Promega was labeled with Cy3-dUTP). Samples with unknown gender where hybridized with labeled male reference DNA.
 
Channel 2
Source name Commercial DNA (Promega)
Organism Homo sapiens
Characteristics gender: F
Growth protocol IPSC (passage between P15 and P33) were cultured on MEF layers in the presence of FGF2
Extracted molecule genomic DNA
Extraction protocol DNA extraction was performed using the DNEasy Mini Kit (Qiagen). DNA was purified using the ChargeSwitch Forensic DNA Purification Kit (Invitrogen, Carlsberg, CA). Concentration and purity of DNA was determined using a Qubit dsDNA BR kit (Life Technologies).
Label Cy3
Label protocol DNA was digested with AluI and RsaI restriction enzymes. Sample DNA was labeled with Cy5-dUTP, whereas sex-matched normal reference DNA from Promega was labeled with Cy3-dUTP). Samples with unknown gender where hybridized with labeled male reference DNA.
 
 
Hybridization protocol Pooled test and reference labeled DNA samples were hybridized for 40 hours at 65°C on Agilent 244K high density oligo microarrays (G4411B, AMADID 014693), and washed with Agilent Wash Buffers 1 & 2 and acetonitril.
Scan protocol Washed arrays were dried using a nitrogen pulse gun and scanned on an Agilent G2565CA scanner (Agilent, Palo Alto, CA) using a resolution of 3µm per pixel and 100% of the photomultiplicator for both signals.
Description Sample name in figure S3a : P3 (F2, III-2) (CD34+)
Data processing Intensity aquisition and data normalization and transformation were performed using Feature Extraction v10.7.3.1 (Agilent Sotware) using default protocol.
 
Submission date Apr 15, 2015
Last update date Aug 06, 2015
Contact name Bastien Job
E-mail(s) bastien.job@gustaveroussy.fr
Phone +330142114211
Organization name Institut Gustave Roussy
Lab Bioinformatics Core Facility
Street address 39 rue Camille Desmoulins
City VILLEJUIF
ZIP/Postal code 94800
Country France
 
Platform ID GPL9777
Series (1)
GSE67938 Germline duplication of ATG2B and GSKIP predisposes to familial essential thrombocythemia rapidly progressing to acute leukemia

Data table header descriptions
ID_REF
VALUE Values correspond to normalized log2(test/ref), where test and ref correspond to pathological samples and Promega commercial normal intensities, respectively.

Data table
ID_REF VALUE
4 0.295823899
5 0.094449721
6 -0.599396997
7 0.063385739
8 0.084342629
9 -0.362158236
10 0.053855811
11 0.121757092
12 -0.684992067
13 0.032412666
14 0.20462178
15 0.122888368
16 -0.067164523
17 -0.534973345
18 -0.47421983
19 -0.038854042
20 -0.193378697
21 0.279194691
22 -0.299170419
23 -0.388825241

Total number of rows: 415056

Table truncated, full table size 7742 Kbytes.




Supplementary file Size Download File type/resource
GSM1659025_US82900153_252185016641_S01_CGH_107_Sep09_1_2.txt.gz 43.4 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap