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Sample GSM1656735 Query DataSets for GSM1656735
Status Public on Feb 19, 2016
Title WTCorticalNeuronsNeurite2
Sample type SRA
Source name WT E18.5 cortical neurons neurite
Organism Mus musculus
Characteristics cell type: E18.5 cortical neurons
compartment: neurite
genotype: wild type
Extracted molecule polyA RNA
Extraction protocol Cells were fractionated using porous membranes into soma and neurite fractions.
For all N2A and CAD samples, libraries were prepared using the standard "dUTP" strand-specific method. For cortical neuron samples, libraries were prepared using the Clontech Low Input Kit.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description WT cortical neurons neurite Rep2
Data processing Reads were aligned to the mm9 genome using either Tophat version 2.0.12 or STAR 2.0.4d
Expression values were calculated using Cufflinks version 2.1.1
Alternative isoform abundances were calculated using MISO
Genome_build: mm9 supplemented with alternative isoforms identified in Merkin et al (Science) 2012
Submission date Apr 13, 2015
Last update date May 15, 2019
Contact name Matthew Taliaferro
Organization name University of Colorado Anschutz Medical Campus
Department Biology
Street address 12801 E 17th Ave, RC1 South Room 10403D
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platform ID GPL19057
Series (1)
GSE67828 Profiling of soma and neurite transcriptomes
BioSample SAMN03481300
SRA SRX993480

Supplementary file Size Download File type/resource
GSM1656735_WTAxon2_fpkm.txt.gz 1.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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