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Sample GSM1652430 Query DataSets for GSM1652430
Status Public on Jun 18, 2015
Title MCF7-luc miR-27b o.e-1
Sample type RNA
Source name miR-27b overexpressing MCF7-luc cell line
Organism Homo sapiens
Characteristics cell line: MCF-7
treatment: miR-27b overexpressing MCF7-luc cell line
tissue: Luminal-type breast cancer cells
gender: female
Treatment protocol Overexpression or knockdown of miR-27b using lentivirus vectors.
Growth protocol The cells were cultured with RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum.
Extracted molecule total RNA
Extraction protocol Total RNA and miRNA were isolated from cells and tumour tissues using the miRNeasy Mini Kit (QIAGEN).
Label Cy3
Label protocol Total RNAs (50 ng) from MCF7-luc derivatives were labelled and amplified using the Low Input Quick Amp labelling kit (Agilent Technologies).
Hybridization protocol The slides were hybridized for 17 h at 65°C, washed with Gene Expression Wash Buffer 1 (Agilent Technologies) for 1 min at room temperature, washed with Gene Expression Wash Buffer 2 (Agilent Technologies) for 1 min at 37 °C, and then air-dried.
Scan protocol The arrays from three independent experiments were analysed using microarray scanner (Agilent Technologies).
Description Luminal-type breast cancer cells
Data processing Gene expression levels were calculated using Feature Extraction version (Agilent Technologies) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Submission date Apr 07, 2015
Last update date Apr 23, 2018
Contact name Ryou-u Takahashi
Organization name National Cancer Center Research Institute
Department Division of Molecular and Cellular Medicine
Street address 1-1, Tsukiji 5-chome, Chuo-ku
City Tokyo
ZIP/Postal code 104-0045
Country Japan
Platform ID GPL16699
Series (1)
GSE67631 Identification of microRNA-27b target genes in breast cancer cell lines
Reanalyzed by GSE113533

Data table header descriptions
VALUE processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
1 1.201102e+005
2 4.312744e+000
3 4.366502e+000
4 4.110304e+003
5 1.094604e+001
6 4.509602e+000
7 6.446449e+000
8 1.032261e+001
9 4.003278e+005
10 2.450884e+002
11 9.225014e+001
12 3.444732e+003
13 1.454434e+002
14 4.774579e+000
15 1.023065e+001
16 1.205166e+001
17 4.189205e+002
18 4.448415e+002
19 2.347846e+002
20 3.144087e+003

Total number of rows: 62976

Table truncated, full table size 1219 Kbytes.

Supplementary file Size Download File type/resource
GSM1652430_US82800151_253949424004_S01_GE1_107_Sep09_2_1.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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