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Sample GSM1634049 Query DataSets for GSM1634049
Status Public on Mar 31, 2018
Title OCI-AML3 cells: Atg5-shRNA inducibly expressed by doxycycline for 6 days-replicate 2
Sample type RNA
Source name Human Atg5-depleted OCI-AML3 cell line
Organism Homo sapiens
Characteristics cell line: OCI-AML3
cell type: human leukemia cells
genotype/variation: stably expressing inducible Atg5 shRNA
treated with: 1 ug/ml doxycycline for 6 days
Treatment protocol Cells were treated with or without 1 ug/ml doxycycline for 6 days
Growth protocol OCI-AML3 cells were cultured in MEM-alpha medium with 20% FBS and 1X penicillin and streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Cy3 and/or Cy5 labeled, amplified antisense complementary RNA (cRNA) targets were prepared from 500 ng of the total RNA using the QuickAmp Labeling kit
Hybridization protocol 850 ug of labeled cRNA was hybridized overnight to Agilent SurePrint G3 Human Gene Expression 8x60k slides
Scan protocol Probe intensities were scanned on the Agilent microarray scanner
Description Atg5-sh+2
Data processing Data were processed into image analysis (.GE1) files using Agilent Feature Extraction Software.
Gene expression analysis was normalized and carried out using BRB-ArrayTools version 3.7.0 Mas5 developed by Dr. Richard Simon and BRB-ArrayTools Development Team.
Submission date Mar 13, 2015
Last update date Mar 31, 2018
Contact name Min Huang
Organization name Stanford Cancer Institute
Department Internal Medicine
Lab Beverly Mitchell
Street address 265 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL13607
Series (1)
GSE66904 Human OCI-AML3 cells: Control vs. Atg5-shRNA or NPM1-shRNA inducibly expressed

Data table header descriptions
VALUE normalized

Data table
1 14.37577248
2 3.321928024
3 3.321928024
4 8.129483223
5 9.906645775
6 7.94471693
7 13.28694057
8 8.416031837
9 3.321928024
10 5.735708237
11 3.321928024
12 9.67512989
13 10.02804947
14 7.494294167
15 14.25106907
16 3.321928024
17 5.5319767
18 3.321928024
19 3.321928024
20 10.62297821

Total number of rows: 62976

Table truncated, full table size 1091 Kbytes.

Supplementary file Size Download File type/resource
GSM1634049_US84703594-Atg5sh+Dox-2.txt.gz 12.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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