|
| Status |
Public on Mar 31, 2018 |
| Title |
OCI-AML3 cells: Atg5-shRNA inducibly expressed by doxycycline for 6 days-replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Human Atg5-depleted OCI-AML3 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: OCI-AML3
cell type: human leukemia cells
genotype/variation: stably expressing inducible Atg5 shRNA
treated with: 1 ug/ml doxycycline for 6 days
|
| Treatment protocol |
Cells were treated with or without 1 ug/ml doxycycline for 6 days
|
| Growth protocol |
OCI-AML3 cells were cultured in MEM-alpha medium with 20% FBS and 1X penicillin and streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Cy3 and/or Cy5 labeled, amplified antisense complementary RNA (cRNA) targets were prepared from 500 ng of the total RNA using the QuickAmp Labeling kit
|
| |
|
| Hybridization protocol |
850 ug of labeled cRNA was hybridized overnight to Agilent SurePrint G3 Human Gene Expression 8x60k slides
|
| Scan protocol |
Probe intensities were scanned on the Agilent microarray scanner
|
| Description |
Atg5-sh+2
|
| Data processing |
Data were processed into image analysis (.GE1) files using Agilent Feature Extraction Software.
Gene expression analysis was normalized and carried out using BRB-ArrayTools version 3.7.0 Mas5 developed by Dr. Richard Simon and BRB-ArrayTools Development Team.
|
| |
|
| Submission date |
Mar 13, 2015 |
| Last update date |
Mar 31, 2018 |
| Contact name |
Min Huang |
| E-mail(s) |
minhuang@stanford.edu
|
| Organization name |
Stanford Cancer Institute
|
| Department |
Internal Medicine
|
| Lab |
Beverly Mitchell
|
| Street address |
265 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE66904 |
Human OCI-AML3 cells: Control vs. Atg5-shRNA or NPM1-shRNA inducibly expressed |
|
