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Sample GSM1629329 Query DataSets for GSM1629329
Status Public on Dec 10, 2015
Title shScramble_rep5
Sample type RNA
 
Source name H1975 cells, shScramble, 24h hypoxia
Organism Homo sapiens
Characteristics cell line: H1975
cell type: lung cancer cells
shRNA: scramble
growth condition: hypoxia for 24hr
Treatment protocol Cells in 10cm dishes were serum starved for 24 hours, then transferred to 1% oxygen for an additional 24 hours. Immediately washed with cold PBS, scraped, and frozen at -80C.
Growth protocol H1975 lung cancer cells with stable knockdown of ACC1, ACLY or ETV4 after 24 hours of serum starvation (0.5% FBS) followed by 24 hours of hypoxia (1% O2).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN's RNeasy Mini Kit.
Label biotin
Label protocol Ambion MessageAmp Premier Kit.
 
Hybridization protocol Affymetrix Human Genome U133A 2.0 Array following the Affymetrix standard protocol.
Scan protocol Affymetrix GeneChip Scanner 7G.
Description SCR-E
Data processing Expression Console was used to generate the RMA file.
 
Submission date Mar 09, 2015
Last update date Dec 10, 2015
Contact name Jen-Tsan Chi
E-mail(s) jentsan.chi@duke.edu
Phone 919-668-4759
Organization name Duke University
Department Molecular Genetics and Microbiology
Street address 101 Science Drive, DUMC 3382
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL571
Series (1)
GSE61286 ACLY and ACC1 Regulate Hypoxia-Induced Apoptosis by Modulating ETV4 via α-ketoglutarate

Data table header descriptions
ID_REF
VALUE RMA normalized data

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.17
AFFX-BioB-M_at 8.66
AFFX-BioB-3_at 8.2
AFFX-BioC-5_at 9.27
AFFX-BioC-3_at 10.29
AFFX-BioDn-5_at 10.95
AFFX-BioDn-3_at 12.29
AFFX-CreX-5_at 13.19
AFFX-CreX-3_at 13.58
AFFX-DapX-5_at 8.69
AFFX-DapX-M_at 9.74
AFFX-DapX-3_at 10.57
AFFX-LysX-5_at 6.89
AFFX-LysX-M_at 7.12
AFFX-LysX-3_at 7.77
AFFX-PheX-5_at 7.77
AFFX-PheX-M_at 7.7
AFFX-PheX-3_at 8.07
AFFX-ThrX-5_at 7.54
AFFX-ThrX-M_at 7.74

Total number of rows: 22277

Table truncated, full table size 348 Kbytes.




Supplementary file Size Download File type/resource
GSM1629329_3478_13258_42405_H1-24-C_HG-U133A+2.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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