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Sample GSM1629322 Query DataSets for GSM1629322
Status Public on Dec 10, 2015
Title shACLY-#1_rep1
Sample type RNA
Source name H1975 cells, shshACLY, 24h hypoxia
Organism Homo sapiens
Characteristics cell line: H1975
cell type: lung cancer cells
growth condition: hypoxia for 24hr
Treatment protocol Cells in 10cm dishes were serum starved for 24 hours, then transferred to 1% oxygen for an additional 24 hours. Immediately washed with cold PBS, scraped, and frozen at -80C.
Growth protocol H1975 lung cancer cells with stable knockdown of ACC1, ACLY or ETV4 after 24 hours of serum starvation (0.5% FBS) followed by 24 hours of hypoxia (1% O2).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN's RNeasy Mini Kit.
Label biotin
Label protocol Ambion MessageAmp Premier Kit.
Hybridization protocol Affymetrix Human Genome U133A 2.0 Array following the Affymetrix standard protocol.
Scan protocol Affymetrix GeneChip Scanner 7G.
Description ACLY-283-A
Data processing Expression Console was used to generate the RMA file.
Submission date Mar 09, 2015
Last update date Dec 10, 2015
Contact name Jen-Tsan Chi
Phone 919-668-4759
Organization name Duke University
Department Molecular Genetics and Microbiology
Street address 101 Science Drive, DUMC 3382
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
Platform ID GPL571
Series (1)
GSE61286 ACLY and ACC1 Regulate Hypoxia-Induced Apoptosis by Modulating ETV4 via α-ketoglutarate

Data table header descriptions
VALUE RMA normalized data

Data table
AFFX-BioB-5_at 8.78
AFFX-BioB-M_at 9.11
AFFX-BioB-3_at 8.73
AFFX-BioC-5_at 9.77
AFFX-BioC-3_at 10.71
AFFX-BioDn-5_at 11.23
AFFX-BioDn-3_at 12.79
AFFX-CreX-5_at 13.41
AFFX-CreX-3_at 13.77
AFFX-DapX-5_at 8.71
AFFX-DapX-M_at 10.03
AFFX-DapX-3_at 10.81
AFFX-LysX-5_at 6.84
AFFX-LysX-M_at 7.18
AFFX-LysX-3_at 8
AFFX-PheX-5_at 7.83
AFFX-PheX-M_at 7.9
AFFX-PheX-3_at 8.2
AFFX-ThrX-5_at 7.63
AFFX-ThrX-M_at 8.01

Total number of rows: 22277

Table truncated, full table size 347 Kbytes.

Supplementary file Size Download File type/resource
GSM1629322_3471_13731_43139_ACLY-283-HYP-24h-B_HG-U133A+2.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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