|
Status |
Public on Sep 28, 2015 |
Title |
AG1BM4_2 (gene) |
Sample type |
RNA |
|
|
Source name |
Mixed RNA of both (1:4)
|
Organism |
Homo sapiens |
Characteristics |
sample type: 1:4 mixture of AGO and BMO
|
Extracted molecule |
total RNA |
Extraction protocol |
Samples used in the study are commercially available RNAs derived from mixture of multiple cell lines and RNAs extracted from patient bone marrow specimens
|
Label |
biotin
|
Label protocol |
100 ng of starting RNAs were amplified with Ambion WT-plus amplification kit and 5,000 ng of biotin-labeled cDNAs were hybridized.
|
|
|
Hybridization protocol |
Labeled cDNAs were hybridized onto Affymetrix Human Transcriptome Arrays v2.0 (HTA2.0) following Affymetrix standard protocols.
|
Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix).
|
Description |
replicate_2
|
Data processing |
Raw data were processed with the Expression Console (Affymetrix) for background correction and normalization, and both probeset (exon) and gene (transcript cluster) level data were generated.
|
|
|
Submission date |
Mar 06, 2015 |
Last update date |
Sep 28, 2015 |
Contact name |
Jinsheng Yu |
E-mail(s) |
jyu@wustl.edu
|
Organization name |
Washington University School of Medicine
|
Department |
Genetics
|
Lab |
GTAC Lab
|
Street address |
660 S. Euclid Ave.
|
City |
St. Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL17586 |
Series (2) |
GSE66648 |
Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology (Affymetrix_HTA2.0) |
GSE66649 |
Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology |
|
Relations |
Alternative to |
GSM1627000 (exon-level analysis) |