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Sample GSM1626580 Query DataSets for GSM1626580
Status Public on Sep 28, 2015
Title BMO
Sample type RNA
Source name Original Bone Marrow RNA
Organism Homo sapiens
Characteristics sample type: pure bone marrow RNA (BMO)
Extracted molecule total RNA
Extraction protocol Samples used in the study are commercially available RNAs derived from mixture of multiple cell lines and RNAs extracted from patient bone marrow specimens
Label Cy5
Label protocol 200 ng of starting RNAs were amplified with MessageAmp™ II aRNA Amplification Kit, 3,000 ng of amplified aRNA were labeled with Kreatech ULS™ Fluorescent Labeling Kit (with Cy5).
Hybridization protocol 2,000 ng of cy5-labeled aRNA were hybridized following Agilent standard hybridization protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 4x44k array slides.
Data processing The scanned images were analyzed with Feature Extraction Software 11.5 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Data values in Matrix sheet are Feature Extraction generated rProcessedSignal in linear scale without normalization (control probes are removed from the matrix table, so the total data lines are 43376).
Submission date Mar 06, 2015
Last update date Sep 28, 2015
Contact name Jinsheng Yu
Organization name Washington University School of Medicine
Department Genetics
Lab GTAC Lab
Street address 660 S. Euclid Ave.
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platform ID GPL4133
Series (2)
GSE66626 Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology (Agilent_4x44K)
GSE66649 Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology

Data table header descriptions
VALUE rProcessedSignal

Data table
12 5444.294
13 67.38684
14 1135.428
15 97.58255
16 2739.199
17 433.7332
18 186.3625
19 28473.93
20 116.6954
21 61.6377
22 73252.31
23 137.776
24 3659.764
25 490.2474
26 78.88507
27 390.8506
28 68.97395
29 62.71345
30 416.2856
31 135.776

Total number of rows: 43376

Table truncated, full table size 620 Kbytes.

Supplementary file Size Download File type/resource
GSM1626580_251485040340_S01_GE1_107_Sep09_red_only_1_1.txt.gz 6.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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