NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1626578 Query DataSets for GSM1626578
Status Public on Sep 28, 2015
Title AG1BM64_1
Sample type RNA
 
Source name Mixed RNA of both (1:64)
Organism Homo sapiens
Characteristics sample type: 1:64 mixture of AGO and BMO
Extracted molecule total RNA
Extraction protocol Samples used in the study are commercially available RNAs derived from mixture of multiple cell lines and RNAs extracted from patient bone marrow specimens
Label Cy5
Label protocol 200 ng of starting RNAs were amplified with MessageAmp™ II aRNA Amplification Kit, 3,000 ng of amplified aRNA were labeled with Kreatech ULS™ Fluorescent Labeling Kit (with Cy5).
 
Hybridization protocol 2,000 ng of cy5-labeled aRNA were hybridized following Agilent standard hybridization protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 4x44k array slides.
Data processing The scanned images were analyzed with Feature Extraction Software 11.5 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Data values in Matrix sheet are Feature Extraction generated rProcessedSignal in linear scale without normalization (control probes are removed from the matrix table, so the total data lines are 43376).
 
Submission date Mar 06, 2015
Last update date Sep 28, 2015
Contact name Jinsheng Yu
E-mail(s) jyu@wustl.edu
Organization name Washington University School of Medicine
Department Genetics
Lab GTAC Lab
Street address 660 S. Euclid Ave.
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL4133
Series (2)
GSE66626 Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology (Agilent_4x44K)
GSE66649 Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology

Data table header descriptions
ID_REF
VALUE rProcessedSignal

Data table
ID_REF VALUE
12 10680.69
13 29.91477
14 2127.738
15 147.5528
16 6789.711
17 640.9663
18 207.7803
19 55768.02
20 96.08153
21 29.60055
22 126484
23 138.7558
24 7214.418
25 1395.332
26 29.51626
27 723.8643
28 29.51957
29 53.36914
30 736.0091
31 61.42695

Total number of rows: 43376

Table truncated, full table size 620 Kbytes.




Supplementary file Size Download File type/resource
GSM1626578_251485040339_S01_GE1_107_Sep09_red_only_1_4.txt.gz 6.6 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap