GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1626577 Query DataSets for GSM1626577
Status Public on Sep 28, 2015
Title AG1BM4_2
Sample type RNA
Source name Mixed RNA of both (1:16)
Organism Homo sapiens
Characteristics sample type: 1:16 mixture of AGO and BMO
Extracted molecule total RNA
Extraction protocol Samples used in the study are commercially available RNAs derived from mixture of multiple cell lines and RNAs extracted from patient bone marrow specimens
Label Cy5
Label protocol 200 ng of starting RNAs were amplified with MessageAmp™ II aRNA Amplification Kit, 3,000 ng of amplified aRNA were labeled with Kreatech ULS™ Fluorescent Labeling Kit (with Cy5).
Hybridization protocol 2,000 ng of cy5-labeled aRNA were hybridized following Agilent standard hybridization protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 4x44k array slides.
Data processing The scanned images were analyzed with Feature Extraction Software 11.5 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Data values in Matrix sheet are Feature Extraction generated rProcessedSignal in linear scale without normalization (control probes are removed from the matrix table, so the total data lines are 43376).
Submission date Mar 06, 2015
Last update date Sep 28, 2015
Contact name Jinsheng Yu
Organization name Washington University School of Medicine
Department Genetics
Lab GTAC Lab
Street address 660 S. Euclid Ave.
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platform ID GPL4133
Series (2)
GSE66626 Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology (Agilent_4x44K)
GSE66649 Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology

Data table header descriptions
VALUE rProcessedSignal

Data table
12 9829.946
13 28.10014
14 2161.292
15 193.7309
16 18807.31
17 536.1028
18 333.2826
19 116945.5
20 79.08436
21 27.94112
22 186305.2
23 156.505
24 11884.56
25 7979.731
26 75.07786
27 1329.812
28 28.03538
29 48.35206
30 3504.233
31 86.37629

Total number of rows: 43376

Table truncated, full table size 620 Kbytes.

Supplementary file Size Download File type/resource
GSM1626577_251485040340_S01_GE1_107_Sep09_red_only_1_2.txt.gz 6.6 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap