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Sample GSM1623444 Query DataSets for GSM1623444
Status Public on Apr 13, 2016
Title Identification of miR-221 target genes in PC3
Sample type RNA
 
Channel 1
Source name control miRNA transfected cell line
Organism Homo sapiens
Characteristics cell line: PC3
cell type: prostate cancer
transfection: control
Treatment protocol microRNA or siRNA transfection using Lipofectamine RNAiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or DMEM.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol following the manufacturer's instructions.
Label Cy3
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
Channel 2
Source name miR-221 transfected cell line
Organism Homo sapiens
Characteristics cell line: PC3
cell type: prostate cancer
transfection: miR-221
Treatment protocol microRNA or siRNA transfection using Lipofectamine RNAiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or DMEM.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol following the manufacturer's instructions.
Label Cy5
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
 
Hybridization protocol Agilent two-color gene expression hyb/wash protocol
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution. Images were quantified using Agilent Feature Extraction software version 10.7.3.1
Description A1-PC3-221_PC3-N2-1
Data processing LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
 
Submission date Mar 04, 2015
Last update date Apr 23, 2018
Contact name Naohiko Seki
E-mail(s) naoseki@faculty.chiba-u.jp
Phone +81-43-226-2971
Organization name Chiba University
Department Functional Genomics
Street address 1-8-1, Inohana
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL16699
Series (1)
GSE66498 Differentially expressed genes after miRNA or siRNA transfection in human cancer cell lines (II)
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 2.266925359e-002
2 3.504265256e-001
3 0.000000000e+000
4 1.663426002e-003
5 1.222167760e-001
6 -3.081816931e-001
7 -4.069770787e-001
8 7.218359018e-001
9 1.224053950e-001
10 -1.611726251e-001
11 1.094462570e-001
12 -1.579119770e-001
13 4.506142719e-002
14 4.316618018e-002
15 0.000000000e+000
16 -3.335918970e-001
17 1.891043713e-001
18 -1.946759807e-001
19 -2.824880139e-001
20 -6.431196646e-002

Total number of rows: 62976

Table truncated, full table size 1425 Kbytes.




Supplementary file Size Download File type/resource
GSM1623444_A1-PC3-221_PC3-N2-1.txt.gz 21.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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