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Sample GSM1619820 Query DataSets for GSM1619820
Status Public on Nov 05, 2015
Title Treated_replicate1
Sample type RNA
 
Source name MDA-MB-231
Organism Homo sapiens
Characteristics cell line: MDA-MB-231
cell type: triple negative breast adenocarcinoma
agent: DETA/NO
Treatment protocol For conducting experiments, cells were grown to 80% confluency and serum starved overnight prior to treatment with 500uM DETA/NO for 24 hours.
Growth protocol MDA-MB-231 human triple negative breast cancer cells were obtained from American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin in a controlled environment (37°C and 5% CO2).
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy kit (Qiagen) as per manufacturer's protocols.
Label Biotin
Label protocol Samples were labeled using the Affymetrix FlashTag™ Biotin HSR RNA Labeling Kit as per manufacturer's protocols.
 
Hybridization protocol The GeneChip® Hybridization, Wash, and Stain Kit was used as per manufacturer’s protocols. The GeneChip® Fluidics Station 450 was used for washing and staining with fluidics script FS450_0002.
Scan protocol Microarray chips were read on the GeneChip® Scanner 3000 7G with AGCC version 4.0.0.1567.
Description Sample name: miRNA_D1
Data processing RMA in Partek
 
Submission date Feb 26, 2015
Last update date Nov 06, 2015
Contact name Douglas David Thomas
E-mail(s) ddthomas@uic.edu
Phone 312-996-6156
Organization name University of Illinois at Chicago
Department Medicinal Chemistry & Pharmacognosy
Street address 900 S Ashland Ave Room 3306
City Chicago
State/province IL
ZIP/Postal code 60607
Country USA
 
Platform ID GPL19117
Series (2)
GSE66323 Nitric oxide regulates gene expression in cancers by controlling histone posttranslational modifications [miRNA-4_0]
GSE66324 Nitric oxide regulates gene expression in cancers by controlling histone posttranslational modifications

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
MIMAT0000001_st 13.49
MIMAT0015091_st 0.92
MIMAT0000002_st 1.34
MIMAT0015092_st 0.9
MIMAT0020301_st 1.3
MIMAT0000003_st 1.19
MIMAT0020302_st 1.15
MIMAT0000004_st 1.01
MIMAT0000005_st 1.5
MIMAT0015093_st 1.38
MIMAT0020303_st 1.68
MIMAT0000006_st 1
MIMAT0020304_st 0.81
MIMAT0000007_st 0.92
MIMAT0015094_st 1.17
MIMAT0000008_st 0.85
MIMAT0020305_st 0.75
MIMAT0000009_st 1
MIMAT0020306_st 0.88
MIMAT0000010_st 1.13

Total number of rows: 36249

Table truncated, full table size 731 Kbytes.




Supplementary file Size Download File type/resource
GSM1619820_THO040914_4_D1_miRNA4_042314_miRNA-4_0_.CEL.gz 792.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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