|
| Status |
Public on Aug 13, 2020 |
| Title |
9345344075_H |
| Sample type |
RNA |
| |
|
| Source name |
HT29 ERb2 TNFa 2h -3
|
| Organism |
Homo sapiens |
| Characteristics |
colon cancer cell line: HT29 with stably expressed Erbeta gene
treatment: 10ng/mL TNFa 2 hours
|
| Treatment protocol |
Cells were treated with either vehicle or 10ng/mL TNFa for either 2 or 24 hours before RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using EZNA Total RNA Kit I, from Omega
|
| Label |
Cy3
|
| Label protocol |
cRNA were prepared and labeled with Cy3 dye using Illumina TotalPrep-96RNA Amplification Kit and Illumina Whole-Genome Gene Expression Direct Hybridisation Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
Array data were normalized and analyzed using R program, lumi and limma packages.
|
| |
|
| Submission date |
Feb 17, 2015 |
| Last update date |
Aug 13, 2020 |
| Contact name |
Cecilia Williams |
| E-mail(s) |
cecilia.williams@scilifelab.se, cwil@kth.se, cecilia.williams@ki.se
|
| Organization name |
KTH Royal Institute of Technology
|
| Department |
Protein Science
|
| Lab |
SciLifeLab
|
| Street address |
Tomtebodav 23
|
| City |
Solna |
| ZIP/Postal code |
171 21 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE65979 |
ERbeta modulation of NFkB pathway in colon cancer cell lines SW480 and HT29 |
|
