|
Status |
Public on Aug 13, 2020 |
Title |
9345344071_J |
Sample type |
RNA |
|
|
Source name |
HT29 mock2 TNFa 2h -2
|
Organism |
Homo sapiens |
Characteristics |
colon cancer cell line: HT29 treatment: 10ng/mL TNFa 2 hours
|
Treatment protocol |
Cells were treated with either vehicle or 10ng/mL TNFa for either 2 or 24 hours before RNA extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using EZNA Total RNA Kit I, from Omega
|
Label |
Cy3
|
Label protocol |
cRNA were prepared and labeled with Cy3 dye using Illumina TotalPrep-96RNA Amplification Kit and Illumina Whole-Genome Gene Expression Direct Hybridisation Kit
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2
|
Data processing |
Array data were normalized and analyzed using R program, lumi and limma packages.
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|
|
Submission date |
Feb 17, 2015 |
Last update date |
Aug 13, 2020 |
Contact name |
Cecilia Williams |
E-mail(s) |
cecilia.williams@scilifelab.se, cwil@kth.se, cecilia.williams@ki.se
|
Organization name |
KTH Royal Institute of Technology
|
Department |
Protein Science
|
Lab |
SciLifeLab
|
Street address |
Tomtebodav 23
|
City |
Solna |
ZIP/Postal code |
171 21 |
Country |
Sweden |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE65979 |
ERbeta modulation of NFkB pathway in colon cancer cell lines SW480 and HT29 |
|