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Sample GSM1606394 Query DataSets for GSM1606394
Status Public on Feb 10, 2017
Title M+CM
Sample type RNA
 
Source name αMHC+ mature cardiomyocytes
Organism Mus musculus
Characteristics cell type: embryonic stem cells
time point: day 10.5
Treatment protocol CsAYTE was treated whenever medium was changed. DMSO was treated as a control vehicle.
Growth protocol For induction and differentiation of Flk1+ MPC, sfPCB, PCB, and M+CM, mouse PSC were cultured in differentiation medium without leukemia inhibitory factor.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions.
Label Cy3
Label protocol Amplified and labeled cRNA was purified on cRNA Cleanup Module (Agilent Technology) according to the manufacturer’s protocol. Labeled cRNA target was quantified using ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).
 
Hybridization protocol After checking labeling efficiency, fragmentation of cRNA was performed by adding 10X blocking agent and 25X fragmentation buffer and incubating at 60oC for 30 min. The fragmented cRNA was resuspended with 2X hybridization buffer and directly pipetted onto assembled Agilent’s Mouse Oligo Microarray (44K). The arrays hybridized at 65oC for 17 hours using Agilent Hybridization oven (Agilent Technology, USA). The hybridized microarrays were washed as the manufacturer’s washing protocol (Agilent Technology, USA).
Scan protocol The hybridized images were scanned using Agilent’s DNA microarray scanner and quantified with Feature Extraction Software (Agilent Technology, Palo Alto, CA).
Description Gene expression at day 10.5
Data processing All data normalization and further analysis were performed using GeneSpringGX 7.3 (Agilent Technology, USA).
 
Submission date Feb 10, 2015
Last update date Feb 10, 2017
Contact name Gou Young Koh
E-mail(s) gykoh@kaist.ac.kr
Phone 82-42-350-2638
Organization name KAIST
Street address 291 Daehark-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 305-701
Country South Korea
 
Platform ID GPL11202
Series (1)
GSE65791 Generation of PDGFRα+ Cardioblasts from Pluripotent Stem Cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity (Global normalization)

Data table
ID_REF VALUE
A_51_P100034 11.87629784
A_51_P100174 7.679029627
A_51_P100208 4.330720443
A_51_P100289 14.09318315
A_51_P100298 6.090576125
A_51_P100309 1.696789747
A_51_P100327 3.697234487
A_51_P100347 5.131934725
A_51_P100519 2.333389383
A_51_P100537 4.1784095
A_51_P100573 9.560042623
A_51_P100624 2.356737954
A_51_P100625 3.198907416
A_51_P100768 1.505984397
A_51_P100776 6.524921212
A_51_P100787 12.26837108
A_51_P100828 14.03226187
A_51_P100852 4.169731106
A_51_P100991 6.525019753
A_51_P100997 6.907760339

Total number of rows: 39429

Table truncated, full table size 981 Kbytes.




Supplementary file Size Download File type/resource
GSM1606394_M+CM.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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