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Sample GSM1606393 Query DataSets for GSM1606393
Status Public on Feb 10, 2017
Title Flk1+MPC
Sample type RNA
Source name Flk1+ MPC as cardiovascular progenitors
Organism Mus musculus
Characteristics cell type: embryonic stem cells
time point: day 4.5
Treatment protocol CsAYTE was treated whenever medium was changed. DMSO was treated as a control vehicle.
Growth protocol For induction and differentiation of Flk1+ MPC, sfPCB, PCB, and M+CM, mouse PSC were cultured in differentiation medium without leukemia inhibitory factor.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions.
Label Cy3
Label protocol Amplified and labeled cRNA was purified on cRNA Cleanup Module (Agilent Technology) according to the manufacturer’s protocol. Labeled cRNA target was quantified using ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).
Hybridization protocol After checking labeling efficiency, fragmentation of cRNA was performed by adding 10X blocking agent and 25X fragmentation buffer and incubating at 60oC for 30 min. The fragmented cRNA was resuspended with 2X hybridization buffer and directly pipetted onto assembled Agilent’s Mouse Oligo Microarray (44K). The arrays hybridized at 65oC for 17 hours using Agilent Hybridization oven (Agilent Technology, USA). The hybridized microarrays were washed as the manufacturer’s washing protocol (Agilent Technology, USA).
Scan protocol The hybridized images were scanned using Agilent’s DNA microarray scanner and quantified with Feature Extraction Software (Agilent Technology, Palo Alto, CA).
Description Gene expression at day 4.5
Data processing All data normalization and further analysis were performed using GeneSpringGX 7.3 (Agilent Technology, USA).
Submission date Feb 10, 2015
Last update date Feb 10, 2017
Contact name Gou Young Koh
Phone 82-42-350-2638
Organization name KAIST
Street address 291 Daehark-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 305-701
Country South Korea
Platform ID GPL11202
Series (1)
GSE65791 Generation of PDGFRα+ Cardioblasts from Pluripotent Stem Cells

Data table header descriptions
VALUE Normalized signal intensity (Global normalization)

Data table
A_51_P100034 10.91078512
A_51_P100174 12.64183534
A_51_P100208 1.820961125
A_51_P100289 13.05152913
A_51_P100298 9.435304176
A_51_P100309 1.702973395
A_51_P100327 4.801599159
A_51_P100347 5.174701623
A_51_P100519 1.788126062
A_51_P100537 4.347528395
A_51_P100573 9.826203025
A_51_P100624 2.875219583
A_51_P100625 6.110784038
A_51_P100768 2.253762712
A_51_P100776 4.886904437
A_51_P100787 12.89105526
A_51_P100828 11.97655514
A_51_P100852 4.550519137
A_51_P100991 7.015256696
A_51_P100997 6.73148446

Total number of rows: 39429

Table truncated, full table size 981 Kbytes.

Supplementary file Size Download File type/resource
GSM1606393_Flk1+MPC.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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