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Sample GSM1603759 Query DataSets for GSM1603759
Status Public on Jun 01, 2016
Title Relapsing_DLBCL_primary (case9)
Sample type genomic
 
Channel 1
Source name FFPE tissue biopsy
Organism Homo sapiens
Characteristics disease state: Diffuse large B cell lymphoma (DLBCL)
specimen: Relapsing DLBCL primary
case: 9
Treatment protocol na
Growth protocol na
Extracted molecule genomic DNA
Extraction protocol H&E- and CD20-stained sections of FFPE blocks or fresh-frozen tissue were reviewed to evaluate tumor content. Only tissues with >70% tumor content were used. Depending on the distribution of tumor cells, 25µm-thick sections were cut or punches were taken from the tumor-rich tissue areas. Prior to overnight Proteinase K digestion, paraffin sections were deparaffinized and rehydrated by serial xylene and ethanol washes, respectively. Genomic DNA (gDNA) was extracted by automation using Maxwell® 16 FFPE plus LEV DNA Purification Kit. gDNA from samples with extremely scarce tumor material was isolated by the phenol-chloroform extraction as described previously22. Yields were quantified by the Qubit assay.
Label cy5
Label protocol A 100-500 nanogram aliquot of each sample and reference DNA was heat digested then labeled with a fluorescent nucleotide (Cy5 for tumor and Cy3 for normal reference) using the BioPrime labeling kit (Invitrogen)according to manufacturer's protocol.
 
Channel 2
Source name Reference pooled 46XX
Organism Homo sapiens
Characteristics tissue: pooled normal (46,XX) reference (Promega)
Treatment protocol na
Growth protocol na
Extracted molecule genomic DNA
Extraction protocol H&E- and CD20-stained sections of FFPE blocks or fresh-frozen tissue were reviewed to evaluate tumor content. Only tissues with >70% tumor content were used. Depending on the distribution of tumor cells, 25µm-thick sections were cut or punches were taken from the tumor-rich tissue areas. Prior to overnight Proteinase K digestion, paraffin sections were deparaffinized and rehydrated by serial xylene and ethanol washes, respectively. Genomic DNA (gDNA) was extracted by automation using Maxwell® 16 FFPE plus LEV DNA Purification Kit. gDNA from samples with extremely scarce tumor material was isolated by the phenol-chloroform extraction as described previously22. Yields were quantified by the Qubit assay.
Label cy3
Label protocol A 100-500 nanogram aliquot of each sample and reference DNA was heat digested then labeled with a fluorescent nucleotide (Cy5 for tumor and Cy3 for normal reference) using the BioPrime labeling kit (Invitrogen)according to manufacturer's protocol.
 
 
Hybridization protocol Labeled DNAs were hybridized in an ozone free environment in a rotisserie oven at 25 rpm for at least 24 hours then washed according to array supplier's (Agilent)protocol
Scan protocol All arrays were scanned using an Agilent 2565C Scanner and default settings for CGH.
Description BIP_case9_primary
Data processing Data was extracted from the TIFF files using Agilent FE 10.5. The data quality was assessed using the QC Report output in F.E. 10.5. All arrays that passed the experimental Q.C. were then visulaized and analyzed using Agilent Genomic Workbench v7.0 and the ADM2 algorithm.
 
Submission date Feb 06, 2015
Last update date Jun 01, 2016
Contact name Darius Juskevicius
E-mail(s) JuskeviciusD@uhbs.ch
Organization name Institute of Pathology
Department Molecuar Pathology
Street address Schoenbeinstr. 40
City Basel
ZIP/Postal code 4031
Country Switzerland
 
Platform ID GPL10150
Series (1)
GSE65720 Clonal relationship of relapsing diffuse large B-cell lymphoma

Data table header descriptions
ID_REF
VALUE normalized log2 ratios (sample/reference)

Data table
ID_REF VALUE
A_16_P15000916 -0.44549167
A_18_P10001325 -1.2296045
A_18_P10001390 0.096125364
A_18_P10001457 -0.33973953
A_18_P10001545 0.10152334
A_16_P15001543 0.45053986
A_16_P15001594 -0.3861356
A_16_P00000099 0.3246637
A_16_P00000136 -0.001030538
A_16_P15001756 -0.088498026
A_16_P00000179 0.19358182
A_16_P00000195 -0.51172125
A_16_P15001886 0.078258455
A_16_P00000237 -0.5238723
A_16_P15001967 -0.023957107
A_16_P00000285 0.05680492
A_16_P00000311 0.10597327
A_16_P15002151 0.09241341
A_18_P17422640 0.36190408
A_16_P00000357 0.108857736

Total number of rows: 169293

Table truncated, full table size 4259 Kbytes.




Supplementary file Size Download File type/resource
GSM1603759_rel_pri_case9.txt.gz 18.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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