GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1603267 Query DataSets for GSM1603267
Status Public on Feb 07, 2015
Title NDV-6h-Rep2
Sample type RNA
Source name HEK293T_NDV-6h
Organism Homo sapiens
Characteristics cell line: HEK293T
infected with: NDV-LasoTa for 6hrs
Extracted molecule total RNA
Extraction protocol Small RNA extraction using miRVana miRNA isolation Kit.
Label biotin
Label protocol omplex probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA)
Hybridization protocol 500 ng RNA were hybridised for 16h at 60 rpm at 48°C on Affymetrix GeneChip miRNA 4.0 Array and scanned by Affymetrix 3000 7G.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description SAMPLE 4.rma-dabg-Signal
Data processing Scanned images of microarray chips were analyzed using Affymetrix Expression Console with the default RMA settings. Raw data were processed and Nromalized on basis of organism human using RMA algorithm. The normalized data (CHP files) processed for the differential miRNA analysis in Affymetrix TAC software.
Submission date Feb 06, 2015
Last update date Feb 07, 2015
Contact name Himanshu Kumar
Organization name IISER Bhopal
Department Biological Sciences
Lab 215
Street address Indore bypass road
City Bhopal
State/province MADHYA PRADESH
ZIP/Postal code 462066
Country India
Platform ID GPL19117
Series (1)
GSE65694 MicroRNA profiling in HEK293T cells after NDV infection

Data table header descriptions
VALUE RMA Signal Intensity

Data table
MIMAT0000001_st 10.5451
MIMAT0015091_st 0.7460611
MIMAT0000002_st 0.6699271
MIMAT0015092_st 4.700114
MIMAT0020301_st 0.4638548
MIMAT0000003_st 0.8017487
MIMAT0020302_st 1.150027
MIMAT0000004_st 0.6972198
MIMAT0000005_st 2.089958
MIMAT0015093_st 2.133884
MIMAT0020303_st 0.2831483
MIMAT0000006_st 0.8904378
MIMAT0020304_st 0.6857091
MIMAT0000007_st 1.064956
MIMAT0015094_st 2.574262
MIMAT0000008_st 0.5755885
MIMAT0020305_st 0.4133364
MIMAT0000009_st 0.890438
MIMAT0020306_st 0.5809805
MIMAT0000010_st 0.4649943

Total number of rows: 36249

Table truncated, full table size 892 Kbytes.

Supplementary file Size Download File type/resource
GSM1603267_Sample4.CEL.gz 802.5 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap