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Sample GSM1603265 Query DataSets for GSM1603265
Status Public on Feb 07, 2015
Title Uninfected-Rep2
Sample type RNA
 
Source name HEK293T_uninfected
Organism Homo sapiens
Characteristics cell line: HEK293T
infected with: none (uninfected)
Extracted molecule total RNA
Extraction protocol Small RNA extraction using miRVana miRNA isolation Kit.
Label biotin
Label protocol omplex probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA)
 
Hybridization protocol 500 ng RNA were hybridised for 16h at 60 rpm at 48°C on Affymetrix GeneChip miRNA 4.0 Array and scanned by Affymetrix 3000 7G.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description SAMPLE 2.rma-dabg-Signal
Data processing Scanned images of microarray chips were analyzed using Affymetrix Expression Console with the default RMA settings. Raw data were processed and Nromalized on basis of organism human using RMA algorithm. The normalized data (CHP files) processed for the differential miRNA analysis in Affymetrix TAC software.
 
Submission date Feb 06, 2015
Last update date Feb 07, 2015
Contact name Himanshu Kumar
E-mail(s) hkumar@iiserb.ac.in
Organization name IISER Bhopal
Department Biological Sciences
Lab 215
Street address Indore bypass road
City Bhopal
State/province MADHYA PRADESH
ZIP/Postal code 462066
Country India
 
Platform ID GPL19117
Series (1)
GSE65694 MicroRNA profiling in HEK293T cells after NDV infection

Data table header descriptions
ID_REF
VALUE RMA Signal Intensity

Data table
ID_REF VALUE
MIMAT0000001_st 10.08033
MIMAT0015091_st 0.4537316
MIMAT0000002_st 1.038586
MIMAT0015092_st 4.392128
MIMAT0020301_st 0.795939
MIMAT0000003_st 0.6661541
MIMAT0020302_st 1.482112
MIMAT0000004_st 0.8519238
MIMAT0000005_st 1.842462
MIMAT0015093_st 2.310123
MIMAT0020303_st 0.5979877
MIMAT0000006_st 1.707383
MIMAT0020304_st 0.2405693
MIMAT0000007_st 1.01259
MIMAT0015094_st 1.415444
MIMAT0000008_st 1.091666
MIMAT0020305_st 0.2017246
MIMAT0000009_st 1.935814
MIMAT0020306_st 0.3684399
MIMAT0000010_st 0.62318

Total number of rows: 36249

Table truncated, full table size 893 Kbytes.




Supplementary file Size Download File type/resource
GSM1603265_Sample2.CEL.gz 804.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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